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In direct antigen testing, the reaction is generally seen with the


A) electron microscope.
B) naked eye.
C) light microscope.
D) API 20E miniature identification system.
E) PCR reaction.

F) A) and E)
G) B) and E)

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Which test is used to measure the IgE present in allergic patients?


A) fluorescent antibody tests
B) Rapid Plasma Reagin (RPR) test
C) Weil-Felix reaction
D) RIST
E) ELISA

F) C) and D)
G) A) and E)

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Sputum is obtained by


A) swabbing the mouth to sample saliva.
B) a clean catch urine sample.
C) the patient coughing up mucus from the lower respiratory system.
D) a spinal tap above the chest.
E) a throat swab.

F) All of the above
G) C) and D)

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Whole antigens are detected in which type of test?


A) cross-reactions
B) agglutination
C) precipitation
D) specificity
E) sensitivity

F) None of the above
G) A) and B)

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Which of the following is involved in the in vitro diagnostic testing of serum?


A) mycology
B) hematology
C) serology
D) histology
E) virology

F) A) and E)
G) D) and E)

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Test systems involving which of the following has greatly improved the specificity of immunological tests?


A) Monoclonal antibodies
B) Electronics
C) Enzymes
D) Radioactivity
E) All of these choices are correct.

F) A) and D)
G) A) and B)

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Select the methods below that can be used for identifying the causative agent of a viral infection.Check all that apply.


A) cultivation of the virus from patient samples in cell/tissue cultures
B) detection of antibodies to the virus in a patient's serum
C) observation of the patient's symptoms
D) gram stain of patient specimens
E) amplification and detection of viral nucleic acid in patient specimens
F) culture of virus from patient specimens in enriched agar and broth media
G) direct observation of viral-infected cells using fluorescent-antibody staining

H) B) and G)
I) C) and G)

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When antibodies or other substances in serum cross-react with the test reagents, a false positive result can occur.

A) True
B) False

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When serum proteins are separated by electrophoresis and then antibodies specific for the serum proteins are placed in a parallel trough in order to form reaction arcs for each protein, the test is called


A) Ouchterlony double diffusion.
B) Western Blot.
C) immunelectrophoresis.
D) radioimmunoassay (RIA) .
E) the Quellung test.

F) A) and E)
G) A) and C)

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Broad general characteristics of bacteria on flowcharts can be misleading for a diagnosis of disease.

A) True
B) False

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Which test uses radioactive isotopes to label antibodies or antigens in order to detect minute amounts of corresponding antigen or antibody?


A) Ouchterlony double diffusion
B) Western Blot
C) immunelectrophoresis
D) radioimmunoassay (RIA)
E) the Quellung test

F) All of the above
G) A) and B)

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Soluble antigens are detected in which type of test?


A) cross-reactions
B) agglutination
C) precipitation
D) specificity
E) sensitivity

F) None of the above
G) A) and C)

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The tuberculin test is an example of an in vivo serological test.

A) True
B) False

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Compare and contrast agglutination and precipitation reactions, and include a description of one test for each type of reaction.

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Agglutination and precipitation reaction...

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Describe three possible sampling sites and methods of collection for clinical laboratories.

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1. Blood Collection from a Vein: One com...

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Minute amounts of a pathogen in a patient sample can be rapidly detected by specifically amplifying and identifying its nucleic acid using PCR.This is an example of


A) an immunologic method.
B) a microscopic method.
C) a phenotypic method.
D) a genetic method.

E) A) and C)
F) A) and B)

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Which test is the confirming test for people who initially tested antibody-positive in the screening ELISA test?


A) Ouchterlony double diffusion
B) Western Blot
C) immunelectrophoresis
D) radioimmunoassay (RIA)
E) the Quellung test

F) A) and B)
G) A) and E)

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The property of a test to detect only a certain antibody or antigen, and not to react with any others, is


A) cross-reactions.
B) agglutination.
C) precipitation.
D) specificity.
E) sensitivity.

F) A) and C)
G) C) and D)

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Serotyping of the pneumococcus based on capsular polysaccharide is performed in


A) Ouchterlony double diffusion.
B) Western Blot.
C) immunelectrophoresis.
D) radioimmunoassay (RIA) .
E) the Quellung test.

F) A) and E)
G) All of the above

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