Question 1

The introduction of DNA into plants for the creation of transgenic plants often uses a plasmid mediated process from which bacterium?

Accepted Answer

A)   Rhizobium legmuninosarum
B)   Pseudomonas syringae
C)   Corynebacterium glutamicum
D)   Xanthomonas campestris
E)   Agrobacterium tumefaciensF) A) and E)
G) A) and D)

Question 2

Cyanobacteria are known to accumulate polyhydroxyalkanoates (PHA).

Accepted Answer

A) True 
 B)False

Question 3

Which of these amino acids are commonly used as supplements in animal feed to promote growth?

Accepted Answer

A)   glycine and glutamate
B)   methionine and lysine
C)   proline and valine
D)   tryptophan and tyrosine
E)   aspartate and histidineF) A) and C)
G) A) and E)

Question 4

The main advantage of using microorganisms for the production of amino acids over chemical synthesis is …

Accepted Answer

A)   cost.
B)   stereospecificity.
C)   increased production yield.
D)   ease of purification.
E)   a single microbe will produce all 20 amino acids.F) D) and E)
G) C) and E)

Question 5

Why is the use of cyanobacteria for the production of bioplastics is an attractive commercial investment?

Accepted Answer

A)   Their PHA are stronger than those found in other bacteria.
B)   The cost of production is less.
C)   Their bioplastics are lighter than other types.
D)   Their bioplastics are more pliable.
E)   The PHA produced are easier to work with than other types.F) B) and E)
G) A) and E)

Question 6

A his-tag or strep-tag is part of the cloning area in expression vectors.How do these sequences function?

Accepted Answer

A)   To increase gene expression from the cloned DNA.
B)   In regulation of expression of the cloned DNA.
C)   As unique restriction enzyme sites to aid in cloning DNA.
D)   Affinity sequences to aid in purification of the recombinant protein.
E)   As signal sequences for the secretion of the recombinant protein.F) A) and D)
G) B) and D)

Question 7

The preferred fuel of choice for the original Model T Ford was gasoline.

Accepted Answer

A) True 
 B)False

Question 8

For optimal expression of a eukaryal gene in bacteria,the expression vector must contain all of the following except one.Which would it NOT include?

Accepted Answer

A)   a bacterial promoter
B)   a bacterial termination sequence
C)   a bacterial ribosomal binding site
D)   a protein to remove the introns
E)   a regulatable promoterF) C) and D)
G) B) and C)

Question 9

Which of these would be an example of a "secondary metabolite" fermentation product?

Accepted Answer

A)   ethanol
B)   acetone
C)   butanol
D)   penicillin
E)   propanolF) B) and D)
G) A) and E)

Question 10

In industry,fermentation must be done anaerobically.

Accepted Answer

A) True 
 B)False

Question 11

Affinity tags often used in modern expression vectors to produce a fusion protein from the gene of interest,aids in recombinant protein purification.

Accepted Answer

A) True 
 B)False

Question 12

What are the advantages of using microbial enzymes for the synthesis of certain chemicals over conventional chemical synthesis?

Accepted Answer

Microbial enzymes offer several advantag...

Question 13

Why are bioplastics often preferred over conventional plastics?

Accepted Answer

A)   Bioplastics are stronger.
B)   Bioplastics are less dense.
C)   Bioplastics are biodegradable.
D)   Bioplastics are cheaper to produce.
E)   Bioplastics are more pliable.F) C) and D)
G) B) and D)

Question 14

Amylase enzymes followed by glucose isomerase are used to make which sweetener?

Accepted Answer

A)   NutraSweet™ (aspartame) 
B)   high fructose corn syrup
C)   saccharin
D)   sucrose
E)   Splenda™ (sucralose) F) B) and D)
G) None of the above

Question 15

Current biofuel research is focused on the use of microalgae for the production of this material.

Accepted Answer

A)   ethanol
B)   methanol
C)   biodiesel
D)   cellulase
E)   methaneF) C) and E)
G) A) and E)

Question 16

Although plant biomass is plentiful for use as biofuel,which major problem needs to be overcome?

Accepted Answer

A)   Increasing the efficiency of breakdown of cellulose into glucose.
B)   Decreasing the cost of production of plant biomass.
C)   Increasing the production of cars that can run on ethanol.
D)   Polluting by-products of the conversion of glucose to ethanol.
E)   Increasing use of biofuels may drive crop prices down.F) C) and D)
G) D) and E)

Question 17

What vitamin is used to treat pernicious anemia?

Accepted Answer

A)   C
B)   B₁
C)   B₁₂
D)   niacin
E)   B₆F) C) and E)
G) C) and D)

Question 18

Expression of eukaryal DNA in bacterial cells requires the insertion of _______ into the plasmid expression vector.

Accepted Answer

The answer of Expression of eukaryal DNA in bacterial cells...

Question 19

What does the term fermentation refer to in an industrial microbiology setting?

Accepted Answer

A)   The anaerobic growth of microbes.
B)   The production of acids and alcohols.
C)   The production of all fermentation products.
D)   The mass culturing of microbes for the production of a product.
E)   A low energy yielding catabolic process.F) None of the above
G) A) and E)

Question 20

Bioprospecting is the search for microorganisms that possess useful features for commercial applications.

Accepted Answer

A) True 
 B)False

Exam 12: Microbial Biotechnology

In industry,fermentation must be done anaerobically.

Correct AnswerverifiedShow Answer

Affinity tags often used in modern expression vectors to produce a fusion protein from the gene of interest,aids in recombinant protein purification.

Correct AnswerverifiedShow Answer

Why is the use of cyanobacteria for the production of bioplastics is an attractive commercial investment?

Correct AnswerverifiedShow Answer

Amylase enzymes followed by glucose isomerase are used to make which sweetener?

Correct AnswerverifiedShow Answer

The main advantage of using microorganisms for the production of amino acids over chemical synthesis is …

Correct AnswerverifiedShow Answer

Which of these would be an example of a "secondary metabolite" fermentation product?

Correct AnswerverifiedShow Answer

A his-tag or strep-tag is part of the cloning area in expression vectors.How do these sequences function?

Correct AnswerverifiedShow Answer

The introduction of DNA into plants for the creation of transgenic plants often uses a plasmid mediated process from which bacterium?

Correct AnswerverifiedShow Answer

The preferred fuel of choice for the original Model T Ford was gasoline.

Correct AnswerverifiedShow Answer

What does the term fermentation refer to in an industrial microbiology setting?

Correct AnswerverifiedShow Answer

What vitamin is used to treat pernicious anemia?

Correct AnswerverifiedShow Answer

Why are bioplastics often preferred over conventional plastics?

Correct AnswerverifiedShow Answer

Bioprospecting is the search for microorganisms that possess useful features for commercial applications.

Correct AnswerverifiedShow Answer

Expression of eukaryal DNA in bacterial cells requires the insertion of _______ into the plasmid expression vector.

Correct AnswerverifiedShow Answer

Cyanobacteria are known to accumulate polyhydroxyalkanoates (PHA).

Correct AnswerverifiedShow Answer

Current biofuel research is focused on the use of microalgae for the production of this material.

Correct AnswerverifiedShow Answer

Which of these amino acids are commonly used as supplements in animal feed to promote growth?

Correct AnswerverifiedShow Answer

Although plant biomass is plentiful for use as biofuel,which major problem needs to be overcome?

Correct AnswerverifiedShow Answer

What are the advantages of using microbial enzymes for the synthesis of certain chemicals over conventional chemical synthesis?

Correct AnswerAnswered by ExamLex AIMicrobial enzymes offer several advantag...View AnswerShow Answer

For optimal expression of a eukaryal gene in bacteria,the expression vector must contain all of the following except one.Which would it NOT include?

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Microbial enzymes offer several advantag...
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