Question 1

Histone modifications do not affect

Accepted Answer

A)   affinity of DNA for histones.
B)   gene expression.
C)   chromatin remodelling.
D)   nucleosome structure.
E)   RNA stability.F) C) and D)
G) A) and E)

Question 2

The control sample hybridized in ChIP-chip is random DNA.

Accepted Answer

A) True 
 B)False

Question 3

DNA microarray probes are

Accepted Answer

A)   made by labelling mRNA.
B)   representative of the entire genome.
C)   usually over 1kb in length.
D)   complementary to cDNA.
E)   radioactively labelled.F) A) and D)
G) A) and E)

Question 4

In case of trisomy 21,the CGH signal ratio would be 3:1 (trisomic:control).

Accepted Answer

A) True 
 B)False

Question 5

Genotypes of approximately 50,000 SNPs can be done simultaneously using DNA microarrays.

Accepted Answer

A) True 
 B)False

Question 6

Which of the following is not a use of the two-colour DNA microarray technique?

Accepted Answer

A)   Shows which mRNAs are overexpressed in a mutant cell,compared to the case of a normal cell
B)   Indicates which mRNAs are not being produced in either of two cell types
C)   Allows identification of a cell's rarely expressed mRNAs (fewer than 10 copies per cell) 
D)   Shows which mRNAs are overexpressed in a normal cell,compared to the case of a mutant cell
E)   Measures relative abundance of mRNA in two samplesF) A) and B)
G) A) and C)

Question 7

Which of the following is a conserved sequence motif that can help define eukaryotic promoters?

Accepted Answer

A)   TGA
B)   GATA box
C)   splicing junction
D)   polyadenylation signal
E)   CAAT boxF) A) and B)
G) B) and E)

Question 8

The control sample hybridized in CGH is haploid DNA.

Accepted Answer

A) True 
 B)False

Question 9

Hybridization is of little or no relevance to

Accepted Answer

A)   CGH.
B)   cDNA microarray analysis.
C)   ChIP-chip analysis.
D)   bisulfite sequencing.
E)   PCR.F) D) and E)
G) A) and B)

Question 10

To measure gene expression,DNA microarray probes can be designed to hybridize to

Accepted Answer

A)   start codons.
B)   introns.
C)   promoter regions.
D)   polyA tails.
E)   3'UTRs.F) B) and D)
G) A) and E)

Question 11

What is the purpose of a ChIP-on-chip experiment?

Accepted Answer

A)   Identify miRNAs and their mRNA targets.
B)   Identify cis-elements to which a transcription factor binds.
C)   Identify transcripts expressed in a genome-wide mutant collection.
D)   Identify rare transcripts that are differentially expressed.
E)   Identify repetitive DNA sequences on chromosomes.F) C) and D)
G) B) and D)

Question 12

The most efficient method for associating human SNPs with complex traits is

Accepted Answer

A)   pedigree analysis.
B)   genome wide association studies.
C)   model organisms.
D)   comparative genome hybridization.
E)   whole genome sequencing.F) All of the above
G) C) and D)

Question 13

Every ATG in the genome is a start codon.

Accepted Answer

A) True 
 B)False

Question 14

Which of the following statements about DNA microarrays is false?

Accepted Answer

A)   They can be made of glass.
B)   They involve DNA sequencing.
C)   Expression is proportional to signal intensity.
D)   Lasers are used in their analysis.
E)   PCR is often used in their productionF) A) and D)
G) A) and C)

Question 15

The total number of SNPs identified in the human genome is approximately 50,000.

Accepted Answer

A) True 
 B)False

Question 16

Design an experiment to identify genes associated with cancer,using expression microarrays.

Accepted Answer

Collect RNA from tumour tissues and norm...

Question 17

The only molecule targeted for methylation in epigenetics is cytosine in CpG islands.

Accepted Answer

A) True 
 B)False

Question 18

What is a DNA microarray?

Accepted Answer

A DNA microarray is a chip to which thou...

Question 19

You label cDNA from a tumour with a red fluorescent dye and you use a green dye to label cDNA from normal tissue.Upon microarray hybridization,you find a spot that has more fluorescent signal than the red signal.What is your conclusion?

Accepted Answer

A)   Increased expression of the gene represented at the green spot is causally related to tumourgenesis.
B)   Transcript of the gene represented at that green spot is less abundant in normal tissue than in the tumour.
C)   Transcripts of all genes are more abundant in normal tissue than in the tumour.
D)   Transcript of the gene represented at that green spot is more abundant in normal tissue than in the tumour.
E)   Decreased expression of the gene represented at the green spot is causally related to tumourgenesis.F) C) and E)
G) A) and C)

Question 20

Housekeeping genes are usually either bright green or bright red in an expression microarray image.

Accepted Answer

A) True 
 B)False

Exam 19: The Genetics of Cancer

Housekeeping genes are usually either bright green or bright red in an expression microarray image.

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Histone modifications do not affect

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DNA microarray probes are

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The total number of SNPs identified in the human genome is approximately 50,000.

Correct AnswerverifiedShow Answer

Genotypes of approximately 50,000 SNPs can be done simultaneously using DNA microarrays.

Correct AnswerverifiedShow Answer

The control sample hybridized in ChIP-chip is random DNA.

Correct AnswerverifiedShow Answer

The control sample hybridized in CGH is haploid DNA.

Correct AnswerverifiedShow Answer

What is the purpose of a ChIP-on-chip experiment?

Correct AnswerverifiedShow Answer

Design an experiment to identify genes associated with cancer,using expression microarrays.

Correct AnswerverifiedCollect RNA from tumour tissues and norm...View AnswerShow Answer

Which of the following is not a use of the two-colour DNA microarray technique?

Correct AnswerverifiedShow Answer

You label cDNA from a tumour with a red fluorescent dye and you use a green dye to label cDNA from normal tissue.Upon microarray hybridization,you find a spot that has more fluorescent signal than the red signal.What is your conclusion?

Correct AnswerverifiedShow Answer

In case of trisomy 21,the CGH signal ratio would be 3:1 (trisomic:control).

Correct AnswerverifiedShow Answer

The only molecule targeted for methylation in epigenetics is cytosine in CpG islands.

Correct AnswerverifiedShow Answer

The most efficient method for associating human SNPs with complex traits is

Correct AnswerverifiedShow Answer

To measure gene expression,DNA microarray probes can be designed to hybridize to

Correct AnswerverifiedShow Answer

Hybridization is of little or no relevance to

Correct AnswerverifiedShow Answer

Every ATG in the genome is a start codon.

Correct AnswerverifiedShow Answer

What is a DNA microarray?

Correct AnswerverifiedA DNA microarray is a chip to which thou...View AnswerShow Answer

Which of the following is a conserved sequence motif that can help define eukaryotic promoters?

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Which of the following statements about DNA microarrays is false?

Correct AnswerverifiedShow Answer

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Collect RNA from tumour tissues and norm...
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A DNA microarray is a chip to which thou...
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