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Assume that a given plasmid vector to be used in a cloning experiment contains 4000 base pairs of DNA.Assume also that the restriction endonuclease Cuj cuts this plasmid at the following sites (starting from an arbitrary zero point): 1000,1500,and 3000.Given complete digestion of the plasmid with the endonuclease so that only linear fragments are produced,what sizes of DNA are expected?

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500 bp,150...

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What is meant by the designation EcoRI?

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one of the first res...

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Over the years,sophisticated plasmid vectors have been developed for use in recombinant DNA technology.List at least two features that have been introduced in particularly useful vectors.

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small size to allow large inse...

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The PCR (polymerase chain reaction) protocol that is currently used in laboratories was facilitated by the discovery of a bacterium called Thermus aquaticus in a hot spring inside Yellowstone National Park,in Wyoming.This organism contains a heat-stable form of DNA polymerase known as Taq polymerase,which continues to function even after it has been heated to 95°C.Why would such a heat-stable polymerase be beneficial in PCR?


A) Each cycle includes a "hot" saturation phase (95°C) ,which allows the primers to anneal to the target DNA.
B) Each cycle includes a "hot" denaturation phase (95°C) ,which serves to sterilize the culture.
C) Each cycle includes a "hot" denaturation phase (95°C) ,which activates the Taq polymerase.
D) Each cycle includes a "hot" denaturation phase (95°C) ,which separates the hydrogen bonds that hold the strands of the template DNA together.
E) More than one of the above are correct.

F) All of the above
G) B) and E)

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Which term refers to the process in which DNA can be introduced into host bacterial cells?

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transforma...

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Name at least two typical characteristics of a DNA cloning plasmid.

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small size,high copy...

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In recombinant DNA technology,a YAC is an enzyme isolated from a large South American,four-legged mammal.

A) True
B) False

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In recombinant DNA technology,YAC,RFLP,and λ have identical uses.

A) True
B) False

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List,in order,the steps usually followed in producing recombinant DNA molecules in a plasmid vector.

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isolation of DNA (foreign and ...

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What is a cDNA molecule?

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A cDNA mol...

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